ABSTRACT
Objective To explore the expression and effect of MP0 and TGF-β1 in ulcerative colitis tissues.Methods The expressions of MPO and TGF-Bl in colonal tissue specimens were determined by immunohistochemistry(two step method)in 37 cases of ulcerative colitis,10 crohng disease and 20 normal mucosa.Results The positive rates of MPO and TGF.B1 were 10％and 20.0％in the normal mucosa,55.0％and 35.O％in crohn's disease,and 89.2％and 86.5％in ulcerative colitis.Expression of MPO and TGF-β1 in the ulcerative colitis were higIIer than that in crohn's disease and normal mucosa(P<0.05),there was correlation between MPO and TGF-β1(r=O.51,P<0.05).Conclusion MPO and FGF-β1 are involved in the pathology of UC,and detection of MPO and TGFβ1 can provide some value to evaluate the conditions of UC.
ABSTRACT
Objective To investigate the efficacy of ultrasound ablation combined with autologous transplantation of bone marrow mononuclear cells in treatment of arteriosclerosis obliterans.Methods Clinical data of 39 patients with arteriosclerosis obliterans treated with ultrasound ablation and autologous transplantation of bone marrow mononuclear cells were analyzed.There were 21 male and 18 female patients,With average age of 63 years.At operation,260 mL of autologous bone marrow was withdrawn,mononuclear cells were separated,and 40 mL supension fluid of stem cells was prepared.Through percutaneous approach or surgical approach the ultrasound ablation catheter was inserted into occluded arterial segments.After ablation was performed,autologous bone marrow mononuclear cells were directly injected into the skeletal muscles of the ischemic leg.Results No complications were found in this group.Postoperatively,rest pain of all the patients was significiently improved and distance of claudication increased.The skin became warm in all legs.Conclusions Ultrasound ablation and autologous transplantation of bone marrow mononuclear cells is a simple,safe and effective method for treating patients with arteriosclerosis obliterans.